Associations between human bacterial pathogens and ARGs are magnified in leachates as landfill ages

Landfills constitute the largest treatment and disposal reservoirs of anthropogenic waste on earth they are continuously releasing antibiotic resistance genes (ARGs) to environment for decades via leachates. Little is known about association between ARGs human bacterial pathogens as a function time. Here, we quantified 10 subtypes ARGs, integrons, (HBPs). Except encoding sulfonamides, beta-lactams, macrolides, aminoglycosides were not related integrons (Spearman, P > 0.05). Over time presence became increasingly more correlated with (Procrustes test; R = 0.81, < 0.05), which primarily identified Proteobacteria , Actinobacteria Firmicutes . Rather than prevalence dynamics community, including increased nitrogen metabolism activity decreased diversity assumed lead magnified HBPs target (Varpart; 13%). • Target associated in Integrons directly enhanced “pathongen-ARGs” associations. Bacteria higher N-metabolizing capacity outcompeted other phyla Pathogens likely acquire less diverse community.